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It involves injecting live sperm into a person's eggs in a laboratory. This procedure can create an embryo (fertilized egg). ICSI is a form of in vitro fertilization (IVF). Healthcare providers most commonly use ICSI when male infertility affects a person's ability to conceive a child. What is IVF and ICSI treatment? IVF (in-vitro fertilisation) and ICSI (intracytoplasmic sperm injection) are assisted reproductive treatment (ART) procedures in which eggs are fertilised outside the body. IVF and ICSI involve a series of steps taken over several weeks.